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Alkaline lipase producing bacteria - Penicillium expansum K40 protoplasts and ...

sof6emdiameterasincubatingcontainerwith3mlofmyeeliaandenzymemixture; myceliapretreatedby0.01mol/LDTTfor30minutes, 0.6mol/LNaC1plus0.3CaCIasosmoticstabilizer; temperaturerange25 Severalformsofprotoplastsreleaseandregenera-tionwereobserved. Theconditionsforprotoplastregenerationwerealsoinvestigated. Theprotoplastwereinoculatedon2agarplatesoverlaidwith0.8agar, afterincubatedat25 ℃ ~ 3O ℃ for40hours, regenerationoccurredandcolonieswereformed. Therateofregenerationover70. Keywords: PenicilliumexpanseK40; protoplast; formationandregeneration ( Editor I hope ) (Continued from page 76 ) StudiesonThermostableandAcidLipaseProducedbyPichiaanomalaZHANGWen-xian, JIANGYong-mei, SHIQiao-qin, wUSong-gang (BioengineeringCollege,[link widoczny dla zalogowanych], Fujl ' mouth TeachersUniversity, Fuzhou350007.China) Abstract: 113lipase -Producingstrainswereselectedfrom1137yeastthroughRhodamineBdyesolidplateassaymethod. Ahighlipase-yieldingstrainof1013wasobtainedbyliquidreselectionfrom24strainswithlargertransparentcyclesInaddition, theoptimallipaseproducingconditionsofstrain1013wereasfollowing: Soybeanmeal2.0H, wheatbran1. O, NH4NO30.4, KH2PO40.3, Mg-SO4 · 7H2O0.25, soybean. | 11.0H. TheoptimalconditionsforlipasewereinitialpH5.8. culture28 ℃ for36h. ThelipaseactivityforhydrolysisofoliveisoptimalatpH4.8andat48 ℃, respectively, thelipasewasstablebelow50 ℃ for40minatpH4.8. Differentmeta1ionsshowedadvefseeffectsonthelipaseactivity. MgandNaenhancedthelipaseactivity, whereasZn, Fe